Keratin 20 (KRT20) Antikörper

Antigen: Keratin 20 (KRT20)

Synonyme: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Klonalität: Monoklonal (KS20-8)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125172

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 20

Immunogen: Cytokeratins isolated from human micro dissected villi of human duodenal mucosa.

Format: Supernatant

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: KS20-8

Spezifität: Cytokeratin 20 is less acidic than the other type I cytokeratins and is of particular interest because of its restricted range of expression. This antibody reacts with cytokeratin polypeptide of 46 kD in immunoblott analyses. Studies have shown that a marked difference exists in the expression of cytokeratin 20 among various carcinomas.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 20 antibody