c-erb-2 Antikörper

Antigen: c-erb-2

Klonalität: Monoklonal (CB11)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125161

Produktbeschreibung

Immunogen: Synthetic peptide representing the internal domain.

Format: Supernatant

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: CB11

Spezifität: This antibody reacts with internal domain of c-erbB-2 oncoprotein. c-erbB-2 is a 185 kD transmembrane protein considered to be a growth factor receptor. About 15 to 30% of invasive ductal carcinoma and 70% of in situ ductal carcinoma are positive for c-erbB-2.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cell membrane

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Rezeptoren, Wachstumsfaktoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human breast carcinoma stained with c-erbB-2 antibody