Vimentin (VIM) Antikörper
| Antigen |
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| Synonyme |
FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime |
| Klonalität |
Monoklonal (V9) |
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Wirt
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Reaktivität
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Human (144), Ratte (Rattus) (66), Maus (60), Huhn (31), Rind (Kuh) (26), Schwein (23), Hund (9), Säugetiere (8), Affe (6), Ziege (5), Hamster (5), Amphibien (4), Potoroo (2), Fisch (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
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Applikation
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Western Blot (WB) (90), Immunfluoreszenz (IF) (68), Immunhistochemie (IHC) (55), Durchflusszytometrie (FACS) (54), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), ELISA (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (26), Immunzytochemie (ICC) (13), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2), Dot Blot (Dot) (1)
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| Produktnummer |
ABIN125156 |
| Menge |
500ul (100ug/ml approximately) (Varianten) |
| Preis |
179.00 $ Zzgl. Versandkosten $35.00
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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Vimentin
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Immunogen
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BALB/C mice were injected with purified vimentin from porcine eye lens.
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Format
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Supernatant
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Isotyp
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IgG1 kappa (Passende Sekundärantikörper)
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Klon
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V9
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Spezifität
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This antibody reacts with the 57 kD intermediate filament protein present in the cells of mesenchymal origin. The antibody does not show any cross-reactivity with other intermediate filament proteins. However, it shows a broad interspecies cross- reactivity.
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Applikationshinweise
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Sarcoma. Cellular localization: Cytoplasm Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
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Konzentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin from the ascites by Protein G chromatography and contains sodium azide as preservative.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Forschungsgebiet
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Zell-/Gewebemarker, Zytoskelett
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "Vimentin (VIM)", Typ "Antikörper" finden
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Wirte
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Kaninchen (89), Maus (63), Huhn (8), Schaf (3), Meerschweinchen (2), Ziege (1)
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Reaktivitäten
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Human (144), Ratte (Rattus) (66), Maus (60), Huhn (31), Rind (Kuh) (26), Schwein (23), Hund (9), Säugetiere (8), Affe (6), Ziege (5), Hamster (5), Amphibien (4), Potoroo (2), Fisch (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
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Applikationen
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Western Blot (WB) (90), Immunfluoreszenz (IF) (68), Immunhistochemie (IHC) (55), Durchflusszytometrie (FACS) (54), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), ELISA (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (26), Immunzytochemie (ICC) (13), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2), Dot Blot (Dot) (1)
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Konjugate
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FITC (9), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
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Epitope
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pSer82 (20), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)
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