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Vimentin (VIM) Antikörper

Antigen

Vimentin (VIM)

Synonyme FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Klonalität Monoklonal (V9)
Wirt
Reaktivität
Alternativen

Human

Applikation
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
Produktnummer ABIN125156
Menge 500ul  (100ug/ml approximately)  (Varianten)
Preis 179.00 $   Zzgl. Versandkosten $35.00
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Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung Vimentin
Immunogen BALB/C mice were injected with purified vimentin from porcine eye lens.
Format Supernatant
Isotyp IgG1 kappa  (Passende Sekundärantikörper)
Klon V9
Spezifität This antibody reacts with the 57 kD intermediate filament protein present in the cells of mesenchymal origin. The antibody does not show any cross-reactivity with other intermediate filament proteins. However, it shows a broad interspecies cross- reactivity.

Anwendungen

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Sarcoma. Cellular localization: Cytoplasm Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
Konzentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin from the ascites by Protein G chromatography and contains sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet Zell-/Gewebemarker, Zytoskelett
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen

Alternativen zu Antigen "Vimentin (VIM)", Typ "Antikörper" finden
Wirte Kaninchen (89), Maus (63), Huhn (8), Schaf (3), Meerschweinchen (2), Ziege (1)
Reaktivitäten Human (144), Ratte (Rattus) (66), Maus (60), Huhn (31), Rind (Kuh) (26), Schwein (23), Hund (9), Säugetiere (8), Affe (6), Ziege (5), Hamster (5), Amphibien (4), Potoroo (2), Fisch (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
Applikationen Western Blot (WB) (90), Immunfluoreszenz (IF) (68), Immunhistochemie (IHC) (55), Durchflusszytometrie (FACS) (54), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), ELISA (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (26), Immunzytochemie (ICC) (13), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2), Dot Blot (Dot) (1)
Konjugate FITC (9), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitope pSer82 (20), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)