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Tumor Protein P53 (TP53) Antikörper

Antigen

Tumor Protein P53 (TP53)

Synonyme p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Klonalität Monoklonal (DO-7)
Wirt
Reaktivität
Alternativen

Human

Applikation
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
Produktnummer ABIN125150
Menge 500ul  (100ug/ml approximately)  (Varianten)
Preis 244.00 $   Zzgl. Versandkosten $35.00
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Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung p53
Immunogen Recombinant human wild type P53 protein expressed in E. coli.
Format Supernatant
Isotyp IgG2b kappa  (Passende Sekundärantikörper)
Klon DO-7
Spezifität This antibody reacts with wild as well as mutant types of P53 protein. It recognizes an epitope in the N-terminus of P53 protein, known to reside between amino acids 35 to 45. This antibody can be demonstrated in 22-76% of the colon, stomach, bladder, breast, lung and testes cancers.

Anwendungen

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast Carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
Konzentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet Transkriptionsfaktoren
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen

Alternativen zu Antigen "Tumor Protein P53 (TP53)", Typ "Antikörper" finden
Wirte Kaninchen (255), Maus (79), Schaf (3)
Reaktivitäten Human (328), Maus (68), Ratte (Rattus) (62), Affe (5), Katze (3), Rind (Kuh) (3), Pferd (3), Hund (2), Hamster (2), Primate (2), Primaten (2), Schaf (2), Kaninchen (1), Xenopus laevis (1)
Applikationen Western Blot (WB) (279), Immunhistochemie (Paraffinschnitte) (IHC (p)) (85), Immunhistochemie (IHC) (81), ELISA (68), Immunfluoreszenz (IF) (61), Immunpräzipitation (IP) (41), Enzyme Immunoassay (EIA) (40), Durchflusszytometrie (FACS) (27), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (26), Immunzytochemie (ICC) (11), Dot Blot (Dot) (8), Transfizierte Zellkultur (TCC) (3), Radioimmunoassay (RIA) (2), Chromatin-Immunopräzipitation (ChiP) (1)
Konjugate FITC (7), Biotin (2), HRP (1), PE (1)
Epitope pSer37 (11), pSer315 (10), pSer33 (9), pSer46 (9), pThr18 (9), pSer392 (8), pSer6 (8), pSer15 (7), AA 371-380 (5), pSer20 (5), pSer32/Ser36 (5), AA 20-25 (4), pSer1232 (4), AA 18-30 (3), C-Term (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer9 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), pSer376 (1), pSer378 (1), pThr55 (1), phospho (1)