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Tumor Protein P53 (TP53) Antikörper
| Antigen | Tumor Protein P53 (TP53) |
| Synonyme | p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53 |
| Klonalität | Monoklonal (DO-7) |
| Wirt |
Alternativen Maus |
| Reaktivität |
Alternativen Human |
| Applikation |
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
|
| Produktnummer | ABIN125150 |
| Menge | 500ul (100ug/ml approximately) (Varianten) |
| Preis | 244.00 $ Zzgl. Versandkosten $35.00 |
| Lieferung nach |
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| Verfügbarkeit | Lieferung in 5 Werktagen |
Produktbeschreibung
| Weitere Bezeichnung | p53 |
| Immunogen | Recombinant human wild type P53 protein expressed in E. coli. |
| Format | Supernatant |
| Isotyp | IgG2b kappa (Passende Sekundärantikörper) |
| Klon | DO-7 |
| Spezifität | This antibody reacts with wild as well as mutant types of P53 protein. It recognizes an epitope in the N-terminus of P53 protein, known to reside between amino acids 35 to 45. This antibody can be demonstrated in 22-76% of the colon, stomach, bladder, breast, lung and testes cancers. |
Anwendungen
| Applikationshinweise | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast Carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100) |
| Konzentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as purified immunoglobulin containing sodium azide as preservative. |
| Lagerung | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Forschungsgebiet | Transkriptionsfaktoren |
| Beschränkungen | Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen. |
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