»
Antikörper
»
anti-Glial Fibrillary Acidic Protein (GFAP) Antikörper
Glial Fibrillary Acidic Protein (GFAP) Antikörper
| Antigen |
|
| Synonyme |
GFAP, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345, gfapl, MGC110485, wu:fb34h11, wu:fk42c12, zgc:110485, etID36982.3, xx:af506734 |
| Klonalität |
Monoklonal (GA5) |
|
Wirt
|
|
|
Reaktivität
|
Human (164), Ratte (Rattus) (69), Schwein (66), Maus (51), Rind (Kuh) (46), Schaf (21), Kaninchen (19), Hund (18), Huhn (13), Meerschweinchen (12), Säugetiere (11), Katze (8), Ziege (3), Vogel (2), Caprinae (2), Hamster (1), Pferd (1), Affe (1), Primate (1)
|
|
Applikation
|
Western Blot (WB) (152), Immunhistochemie (Paraffinschnitte) (IHC (p)) (106), Immunfluoreszenz (IF) (99), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (62), Immunhistochemie (IHC) (46), Immunpräzipitation (IP) (31), Durchflusszytometrie (FACS) (23), Immunzytochemie (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (15), ELISA (Detektionsantikörper) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Fangantikörper) (1), Immunoelectron Microscopy (IEM) (1)
|
| Produktnummer |
ABIN125137 |
| Menge |
500ul (100ug/ml approximately) (Varianten) |
| Preis |
170.00 $ Zzgl. Versandkosten $35.00
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 5 Werktagen |
|
Immunogen
|
BALB/C mice were immunized with glial fibrillary protein from porcine spinal cord.
|
|
Format
|
Supernatant
|
|
Isotyp
|
IgG1 kappa (Passende Sekundärantikörper)
|
|
Klon
|
GA5
|
|
Spezifität
|
This antibody reacts with the 52 kD intermediate filament protein GFAP in brain and spinal cord. It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. This antibody does not react with other intermediate filament proteins.
|
|
Applikationshinweise
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasm Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
|
|
Konzentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.
|
|
Lagerung
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Forschungsgebiet
|
Zell-/Gewebemarker, Zytoskelett, Neurologie
|
|
Beschränkungen
|
Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
|
Alternativen zu Antigen "Glial Fibrillary Acidic Protein (GFAP)", Typ "Antikörper" finden
|
Wirte
|
Maus (150), Kaninchen (82), Huhn (12), Ziege (4), Meerschweinchen (2)
|
|
Reaktivitäten
|
Human (164), Ratte (Rattus) (69), Schwein (66), Maus (51), Rind (Kuh) (46), Schaf (21), Kaninchen (19), Hund (18), Huhn (13), Meerschweinchen (12), Säugetiere (11), Katze (8), Ziege (3), Vogel (2), Caprinae (2), Hamster (1), Pferd (1), Affe (1), Primate (1)
|
|
Applikationen
|
Western Blot (WB) (152), Immunhistochemie (Paraffinschnitte) (IHC (p)) (106), Immunfluoreszenz (IF) (99), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (62), Immunhistochemie (IHC) (46), Immunpräzipitation (IP) (31), Durchflusszytometrie (FACS) (23), Immunzytochemie (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (15), ELISA (Detektionsantikörper) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Fangantikörper) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Konjugate
|
Biotin (6), Cy3 (3), HRP (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitope
|
C-Term (1), internal (1), pSer8 (1)
|