Cytokeratin 10/13 Antikörper

Antigen: Cytokeratin 10/13

Klonalität: Monoklonal (DE-K13)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125129

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 10 & 13

Immunogen: BALB/C mice were immunized with detergent insoluble extract of human epidermis.

Format: Supernatant

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: DE-K13

Spezifität: This antibody recognizes keratin polypeptides of 53 and 56.5KD. It stains suprabasal cells in the epidermis, thymic Hassal's bodies, urothelium and non-cornifying stratified epithelia of vagina, ectocervix, tongue and esophagus. This antibody does not stain intraepithelial neoplasm and invasive squamous cell carcinoma.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is not recommended prior to the immunostaining, as it may reduce the staining. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with Cytokeratin 10/13 antibody