Cytokeratin 10 (KRT10) Antikörper

Antigen: Cytokeratin 10 (KRT10)

Synonyme: K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik

Klonalität: Monoklonal (DE-K10)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125128

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 10

Immunogen: BALB/C mice were immunized with detergent insoluble extract of human epidermis.

Format: Supernatant

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: DE-K10

Spezifität: This antibody recognizes keratin polypeptide of 56.5 kD. It stains suprabasal cells in the epidermis, thymic Hassal's bodies. Simple and glandular epithelia, transitional epithelium and adenocarcinomas are negative with this antibody.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is not recommended prior to the immunostaining, as it may reduce the staining. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human skin stained with Cytokeratin 10 antibody

Publikationen

Publikationen: Chamcheu, Loriue, Akgul et al.: "Characterization of immortalized human epidermolysis bullosa simplex (KRT5) cell lines: trimethylamine N-oxide protects the keratin cytoskeleton against disruptive stress condition." in: Journal of dermatological science, Vol. 53, Issue 3, pp. 198-206, 2009 (PubMed).