Prion Protein (PRNP) Antikörper

Antigen: Prion Protein (PRNP)

Synonyme: CJD, GSS, PrP, ASCR, PRIP, PrPc, CD230, prion, MGC26679, PrP27-30, PrP33-35C

Klonalität: Monoklonal (Clone 3C10)

Wirt: Maus

Reaktivität: Rind (Kuh)

Applikation: Western Blot (WB), ELISA

Produktnummer: ABIN123807

Produktbeschreibung

Weitere Bezeichnung: prion protein

Format: Liquid

Klon: Clone 3C10

Beschreibung: Monoclonal antibody (mAb)3C10 is a murine IgG2a subtype antibody.

Spezifität: MAb 3C10 recognizes the sequence G108 QWNKP113 of the bovine prion protein (human PrP: amino acids 97-102). This sequence near the N-terminal proteinase K digestion site is conserved to some extent in known mammalian prion proteins.

Anwendungen

Applikationshinweise: mAb 3C10 detects recombinant PrPc from cattle, sheep, hamster, mouse and human in Western blots. It also detects the bovine prion protein in immunoblots of brain homogenates of healthy and BSE-affected animals, and the partly refolded recombinant bovine PrP in ELISA applications.

Lagerung: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Prion Protein (PRNP) antibody

Publikationen

Publikationen: Schaller, Fatzer, Stack et al.: "Validation of a western immunoblotting procedure for bovine PrP(Sc) detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE)." in: Acta neuropathologica, Vol. 98, Issue 5, pp. 437-43, 1999 (PubMed).

Kuebler, Oesch, Raeber: "Diagnosis of prion diseases." in: British medical bulletin, Vol. 66, pp. 267-79, 2003 (PubMed).

Kim, Umetani, Matsui et al.: "Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies." in: Virology, Vol. 320, Issue 1, pp. 40-51, 2004 (PubMed).