serine/arginine-Rich Splicing Factor 2 (SRSF2) Antikörper

Antigen: serine/arginine-Rich Splicing Factor 2 (SRSF2)

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB)

Produktnummer: ABIN123803

Produktbeschreibung

Weitere Bezeichnung: Splicing Component, 35 kDa

Format: Liquid

Beschreibung: SC35, a member of SR protein family, is an essential pre-mRNA splicing factor and can function as an essential splicing factor as well as an alternative splicing factor. Phosphorylation on serine residues located within the SR domain directly regulates SC35 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, SC35 has been shown to interact either directly or indirectly interacts with the C-terminal domain (CTD) of the largest subunit of RNA Polymerase II, thereby suggesting a potential role of SC35 in coordinating of transcription and pre-mRNA splicing. alpha-SC35 is an affinity-purified rabbit polyclonal antibody raised against the recombinant SC35 protein.

Spezifität: Alpha-SC35 reacts with the SC35 protein in HeLa nuclear extract by Western blotting.

Anwendungen

Applikationshinweise: Recommended dilution range for Western blot analysis: 1:200 - 1:1000.

Lagerung: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Fu, Maniatis: "Isolation of a complementary DNA that encodes the mammalian splicing factor SC35." in: Science (New York, N.Y.), Vol. 256, Issue 5056, pp. 535-8, 1992 (PubMed).

Zahler, Lane, Stolk et al.: "SR proteins: a conserved family of pre-mRNA splicing factors." in: Genes & development, Vol. 6, Issue 5, pp. 837-47, 1992 (PubMed).

Gui, Tronchere, Chandler et al.: "Purification and characterization of a kinase specific for the serine- and arginine-rich pre-mRNA splicing factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 23, pp. 10824-8, 1994 (PubMed).

Colwill, Pawson, Andrews et al.: "The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution." in: The EMBO journal, Vol. 15, Issue 2, pp. 265-75, 1996 (PubMed).

Mortillaro, Blencowe, Wei et al.: "A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8253-7, 1996 (PubMed).

Misteli, Caceres, Spector: "The dynamics of a pre-mRNA splicing factor in living cells." in: Nature, Vol. 387, Issue 6632, pp. 523-7, 1997 (PubMed).