Positive Cofactor 4 (SUB1) Antikörper

Antigen: Positive Cofactor 4 (SUB1)

Synonyme: P15, PC4, p14, MGC102747

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN123802

Produktbeschreibung

Weitere Bezeichnung: Positive Cofactor 4

Format: Antiserum

Beschreibung: The human PC4 (also called p15) is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators. The function of PC4 is apparently through interactions with transcriptional activators and the basal transcription machinery. It is negatively regulated by casein kinase II phosphorylation both in vivo and in vitro. PC4 strongly binds single stranded DNA and regulates HSSB (RPA)-dependent SV40 DNA replication. Recent studies indicated that PC4 can be acetylated by several histone acetyltransferase. alpha-PC4 is rabbit pol yclonal seum raised against the recombinant full-length PC4 protein.

Spezifität: Alpha-PC4 reacts with the PC4 protein in HeLa nuclear extract by Western blotting.

Anwendungen

Applikationshinweise: Recommended dilution range for Western blot analysis: 1:2000 - 1:5000.

Lagerung: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ge, Zhao, Chait et al.: "Phosphorylation negatively regulates the function of coactivator PC4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 26, pp. 12691-5, 1995 (PubMed).

Ge, Roeder: "Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes." in: Cell, Vol. 78, Issue 3, pp. 513-23, 1994 (PubMed).

Kretzschmar, Kaiser, Lottspeich et al.: "A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators." in: Cell, Vol. 78, Issue 3, pp. 525-34, 1994 (PubMed).

Brandsen, Werten, van der Vliet et al.: "C-terminal domain of transcription cofactor PC4 reveals dimeric ssDNA binding site." in: Nature structural biology, Vol. 4, Issue 11, pp. 900-3, 1997 (PubMed).

Wu, Chiang: "Properties of PC4 and an RNA polymerase II complex in directing activated and basal transcription in vitro." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12492-8, 1998 (PubMed).

Werten, Stelzer, Goppelt et al.: "Interaction of PC4 with melted DNA inhibits transcription." in: The EMBO journal, Vol. 17, Issue 17, pp. 5103-11, 1998 (PubMed).

Ge: "UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions." in: Nucleic acids research, Vol. 28, Issue 2, pp. e3, 1999 (PubMed).