High Mobility Group 1 Antikörper

Antigen: High Mobility Group 1

Synonyme: Hmg1, Ac2-008, MGC93598, MGC93599

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB)

Produktnummer: ABIN123797

Produktbeschreibung

Format: Antiserum

Beschreibung: High mobility group 1 (HMG1) is a 26 k Da highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80?90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG1 is a late mediator of delayed endotoxin lethality by activating downstream cytok ine release.

Spezifität: Alpha-HMG1 reacts with the HMG1 protein in HeLa nuclear extract by Western blotting.

Anwendungen

Applikationshinweise: Recommended dilution range for Western blot analysis: 1:2000 - 1:5000.

Lagerung: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bustin, Lehn, Landsman: "Structural features of the HMG chromosomal proteins and their genes." in: Biochimica et biophysica acta, Vol. 1049, Issue 3, pp. 231-43, 1990 (PubMed).

Bianchi, Beltrame, Paonessa: "Specific recognition of cruciform DNA by nuclear protein HMG1." in: Science (New York, N.Y.), Vol. 243, Issue 4894 Pt 1, pp. 1056-9, 1989 (PubMed).

Ge, Roeder: "The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein." in: The Journal of biological chemistry, Vol. 269, Issue 25, pp. 17136-40, 1994 (PubMed).

Stelzer, Goppelt, Lottspeich et al.: "Repression of basal transcription by HMG2 is counteracted by TFIIH-associated factors in an ATP-dependent process." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4712-21, 1994 (PubMed).

Onate, Prendergast, Wagner et al.: "The DNA-bending protein HMG-1 enhances progesterone receptor binding to its target DNA sequences." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3376-91, 1994 (PubMed).

Zappavigna, Falciola, Helmer-Citterich et al.: "HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation." in: The EMBO journal, Vol. 15, Issue 18, pp. 4981-91, 1996 (PubMed).

Jayaraman, Moorthy, Murthy et al.: "High mobility group protein-1 (HMG-1) is a unique activator of p53." in: Genes & development, Vol. 12, Issue 4, pp. 462-72, 1998 (PubMed).

Zlatanova, van Holde: "Binding to four-way junction DNA: a common property of architectural proteins?" in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 12, Issue 6, pp. 421-31, 1998 (PubMed).

Lu, Peterson, Dasgupta et al.: "Influence of HMG-1 and adenovirus oncoprotein E1A on early stages of transcriptional preinitiation complex assembly." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35006-12, 2000 (PubMed).

Yang, Wang, Tracey: "HMG-1 rediscovered as a cytokine." in: Shock (Augusta, Ga.), Vol. 15, Issue 4, pp. 247-53, 2001 (PubMed).