RAP30, Small Subunit of Human Transcription Factor IIF Antikörper

Antigen: RAP30, Small Subunit of Human Transcription Factor IIF

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN123791

Produktbeschreibung

Format: Liquid

Beschreibung: The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP30) subunits as a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E. coli factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74. alpha-TFIIF(RAP30) is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length RAP30 subunit of the TFIIF complex.

Spezifität: Alpha-TFIIF reacts with the RAP30 protein in HeLa nuclear extract by Western blotting.

Anwendungen

Applikationshinweise: Recommended dilution range for Western blot analysis: 1:200 - 1:1000.

Lagerung: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: McCracken, Greenblatt: "Related RNA polymerase-binding regions in human RAP30/74 and Escherichia coli sigma 70." in: Science (New York, N.Y.), Vol. 253, Issue 5022, pp. 900-2, 1991 (PubMed).

Sopta, Burton, Greenblatt: "Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II." in: Nature, Vol. 341, Issue 6241, pp. 410-4, 1989 (PubMed).

Sopta, Carthew, Greenblatt: "Isolation of three proteins that bind to mammalian RNA polymerase II." in: The Journal of biological chemistry, Vol. 260, Issue 18, pp. 10353-60, 1985 (PubMed).