P34 Subunit of Human Transcription Factor IIEbeta Antikörper

Antigen: P34 Subunit of Human Transcription Factor IIEbeta

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN123789

Produktbeschreibung

Format: Liquid

Beschreibung: The human Transcription Factor IIE (TFIIE) is composed of 56 kDa (alpha) and 34 kDa (beta) subunits and is shown to be a heterotetramer. The 56 kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the preinitiation complex probably concomitant with RNA polymerase II and TFIIF. alpha-TFIIE beta is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p34 subunit of the TFIIE complex.

Spezifität: Alpha-TFIIE beta reacts with the p34 protein in HeLa nuclear extract by Western blotting.

Anwendungen

Applikationshinweise: Recommended dilution range for Western blot analysis: 1:200 - 1:1000.

Lagerung: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Peterson, Inostroza, Maxon et al.: "Structure and functional properties of human general transcription factor IIE." in: Nature, Vol. 354, Issue 6352, pp. 369-73, 1992 (PubMed).

Inostroza, Flores, Reinberg: "Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE." in: The Journal of biological chemistry, Vol. 266, Issue 14, pp. 9304-8, 1991 (PubMed).

Flores, Ha, Reinberg: "Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF." in: The Journal of biological chemistry, Vol. 265, Issue 10, pp. 5629-34, 1990 (PubMed).

Ohkuma, Sumimoto, Horikoshi et al.: "Factors involved in specific transcription by mammalian RNA polymerase II: purification and characterization of general transcription factor TFIIE." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 23, pp. 9163-7, 1991 (PubMed).