Bacillus Anthracis Protective Gen Antikörper

Antigen: Bacillus Anthracis Protective Gen

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN123283

Produktbeschreibung

Swiss-Prot: P13423

Immunogen: Synthetic peptides corresponding to amino acids 238-254 of the Anthrax PA protein (~83 kD)

Format: Serum

Beschreibung: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. PA is secreted from Bacillus anthracis as an 83 kDa (PA83) protein which is cleaved by furin protease, releasing a 20 kDa fragment. The 63 kDa portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects.

Spezifität: This antibody is specific for Anthrax PA. PA is cleaved from the 83 kDa form to the 63 kDa form at amino acids 164-167. Thus the antibody (made against amino acids 250-250) should recognize both the 83 kDa and 63 kDa PA forms, but not the 20 kDa cleavage fragment.

Anwendungen

Applikationshinweise: Western blot: 1:1000. Recommended Positive Control: PA recombinant protein. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of Anthrax PA in recombinant protein using ABIN123283 (lane B) at 1:1000 dilution. Lane A showsthepre-bleed

Publikationen

Publikationen: Collier, Young: "Anthrax toxin." in: Annual review of cell and developmental biology, Vol. 19, pp. 45-70, 2003 (PubMed).