Bacillus anthracis lethal factor (47-65) Antikörper

Antigen: Bacillus anthracis lethal factor (47-65)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN123282

Produktbeschreibung

Swiss-Prot: P15917

Immunogen: Synthetic peptides corresponding to amino acids 47-65 of the Anthrax LF protein (~90 kD)

Format: Serum

Beschreibung: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family (2).

Spezifität: This antibody is specific for Anthrax LF.

Anwendungen

Applikationshinweise: Western blot: 1/1000. Recommended positive control: recombinant protein. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of Anthrax LF in recombinant protein using ABIN123282 (lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

Publikationen

Publikationen: Collier, Young: "Anthrax toxin." in: Annual review of cell and developmental biology, Vol. 19, pp. 45-70, 2003 (PubMed).