Aralkylamine N-Acetyltransferase (AANAT) (pSer206) Antikörper

Antigen: Aralkylamine N-Acetyltransferase (AANAT)

Synonyme: SNAT, AA-NAT

Epitope: pSer206

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN122881

Produktbeschreibung

Weitere Bezeichnung: Serotonin acetylase pSer206

Swiss-Prot: Q64666

Immunogen: Synthetic Peptide: C(195) H T F L R R N S(p) G C(205).

Beschreibung: Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

Spezifität: ABIN122881 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues. ABIN122881 immunizing phosphopeptide (ABIN126468) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (ABIN122880) was affinity purified on resin- coupled non-phosphopeptide (ABIN126467). Phospho-specific carboxy-terminal IgG (ABIN122881) was subsequently isolated on resin-coupled phosphopeptide (ABIN126468). This sequence is completely conserved between rat and mouse AA-NAT protein

Anwendungen

Applikationshinweise: Western blot: 0.5 ug/ml, this antibody detects an ~23 kDa protein representing phosphorylated AA-NAT from rat pineal gland whole protein extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative.

Lagerung: Store the antibody at 2-8°C for one month or (in aliquots)at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Enzyme, Phospho-Antikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot of serotonin AA-NAT in transfected 293T cell lysate using ABIN122881.