Serotonin Acetylase (AANAT) Antikörper

Antigen: Serotonin Acetylase (AANAT)

Synonyme: SNAT, AA-NAT

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN122880

Produktbeschreibung

Weitere Bezeichnung: Serotonin acetylase

Swiss-Prot: Q64666

Immunogen: Synthetic Peptide: C(195) H T F L R R N S G C(205).

Beschreibung: Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation. Alternate Names: AA-NAT.

Spezifität: This antibody detects serotonin N-acetyltransferase (AA-NAT) from rat tissues. ABIN122880 immunizing phosphopeptide (ABIN126468) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (ABIN122880) was affinity purified on resin- coupled non-phosphopeptide (ABIN126467). Phospho-specific carboxy-terminal IgG (ABIN122881) was subsequently isolated on resin-coupled phosphopeptide (ABIN126468). This sequence is completely conserved between rat and mouse AA-NAT protein

Anwendungen

Applikationshinweise: Western Blot: 0.5 ug/ml, detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS containing 0.05% sodium azide as preservative and 1 mg/ml BSA as stabilizer.

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot of serotonin AA-NAT in transfected 293T cell lysate using ABIN122880.