Aralkylamine N-Acetyltransferase (AANAT) (pThr29) Antikörper

Antigen: Aralkylamine N-Acetyltransferase (AANAT)

Synonyme: SNAT, AA-NAT

Epitope: pThr29

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN122879

Produktbeschreibung

Weitere Bezeichnung: Serotonin acetylase pThr29

Swiss-Prot: O88816

Immunogen: Synthetic Peptide: C(24) Q R R H T(p) L P A S(33).

Beschreibung: Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

Spezifität: ABIN122879 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues. ABIN122879 immunizing phosphopeptide (ABIN126466) corresponds to amino-terminal amino acid residues 24-33 of mouse AA-NAT. Total anti-amino-terminal AA-NAT-specific IgG (ABIN122878) was affinity purified on resin-coupled non-phosphopeptide (ABIN126465). Phospho-specific amino-terminal IgG (ABIN122879) was subsequently isolated on resin-coupled phosphopeptide (ABIN126466). This sequence is completely conserved between mouse, rat, and human AA-NAT protein

Anwendungen

Applikationshinweise: Western Blot: 0.5 ug/ml, this antibody detects an ~23 kDa protein representing phospho AA- NAT in rat pineal gland whole protein extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative.

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot of phospho AA-NAT in transfected 293T cell lysate using ABIN122879.