Heat Shock 27kDa Protein 1 (HSPB1) Antikörper

Antigen: Heat Shock 27kDa Protein 1 (HSPB1)

Synonyme: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Western Blot (WB)

Produktnummer: ABIN122714

Produktbeschreibung

Weitere Bezeichnung: HSPB1 / HSP27

Swiss-Prot: P04792

Immunogen: Synthetic Peptide: L(10) L R G P S W D P F R C(21) corresponding to amino acid residues 10-21 from human HSP27.

Beschreibung: HSP27 also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that HSP27 may be a useful marker in classifying various hormone sensitive tumors. Alternate Names: HSP-27.

Spezifität: This antibody detects heat shock protein 27(hsp27) from rat and human tissue tissues. This peptide is 81% conserved in mice, rats, and hamsters. This peptide (ABIN126345) is available for use in Neutralization and Control experiments

Synonyme: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Anwendungen

Applikationshinweise: Western Blot: 0.5 ug/ml, detects a ~27 kDa protein which corresponds to hsp27 from HeLa cells. Immunohistochemistry: 4 ug/ml. Immunocytochemistry: staining of hsp27 in HeLa cells with this antibody results in diffuse cytoplasmic staining before heat shock and diffuse cytoplasmic along with punctate staining patterns after. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative.

Lagerung: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar