SARS N (0) Antikörper

Antigen: SARS N (0)

Klonalität: Monoklonal (Ncap11)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN121338

Produktbeschreibung

Produktmerkmale: Synonyms: SARS-CoV N, Severe acute respiratory syndrome coronavirus Nucleoprotein, Nucleocapsidprotein, Protein N, NC, 9a

Weitere Bezeichnung: SARS N

Gen-ID: 1489678

Swiss-Prot: P59595

Immunogen: Full-length recombinant SARS Nucleocapsid Protein. Remarks: Genbank accession no. NP_828858

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Ncap11

Beschreibung: It has recently been shown that SARS is caused by a human coronavirus. Humancoronaviruses are the major cause of upper respiratory tract illness in humans, such as thecommon cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viralRNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding ofthe viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spikeprotein is the main surface antigen of the coronavirus. The most prominent protein in theculture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. Thissuggests that the nucleocapsid protein is a major immunogen that may be useful for earlydiagnostics.

Spezifität: This antibody is specific for SARS-Nucleocapsid protein. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Western blot analysis: 1/500-1/1000 (Detects a band of ~46 kDa). Immunofluorescence/Immunocytochemistry: 1/500. Recommended Positive Control: Transfected Mouse melanoma cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.5 mg/ml

Buffer: PBS containing 0.2% Gelatin as stabilizer and 0.05% Sodium Azide aspreservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using ABIN121338 at 1:1000.

Transfected HeLa cells were stained with ABIN121338 at 1:500 dilution.

Publikationen

Publikationen: Rota, Oberste, Monroe et al.: "Characterization of a novel coronavirus associated with severe acute respiratory syndrome." in: Science (New York, N.Y.), Vol. 300, Issue 5624, pp. 1394-9, 2003 (PubMed).

Krokhin, Li, Andonov et al.: "Mass spectrometric characterization of proteins from the SARS virus: a preliminary report." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 5, pp. 346-56, 2003 (PubMed).