Heat Shock Protein 65 (HSP65) Antikörper

Antigen: Heat Shock Protein 65 (HSP65)

Synonyme: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60

Klonalität: Monoklonal (4H11)

Wirt: Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN121241

Produktbeschreibung

Weitere Bezeichnung: Heat shock protein 65 / HSP65

Swiss-Prot: P0A520

Immunogen: Purified recombinant M. bovis BCG Hsp65

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 4H11

Beschreibung: Hsp65, a 65 kDa protein isolated from Mycobacterium bovis BCG, is a member of the hsp60 family of heat shock proteins (1,2). Sequence comparison of Hsp65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (1,3). Mycobacterium bovis BCG Hsp65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (4).

Spezifität: This antibody detects ~65 kDa protein, corresponding to the apparent molecular mass of Hsp65 on SDS-PAGE immunoblots.

Anwendungen

Applikationshinweise: Western blot analysis (1 ug/ml). Recommended positive control: M. bovis or M. leprae samples. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS (pH 7.2), 0.1 mM PMSF and 50% glycerol

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf Life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of BCG Hsp65, probed with ABIN121241.

Publikationen

Publikationen: Shinnick, Sweetser, Thole et al.: "The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG." in: Infection and immunity, Vol. 55, Issue 8, pp. 1932-5, 1987 (PubMed).

Davis, Cook, Van et al.: "Polymorphic Bgl II restriction sites of DR alpha demarcate a novel HLA-DR1 antigen." in: Immunogenetics, Vol. 28, Issue 3, pp. 171-81, 1988 (PubMed).

Thole, Keulen, De Bruyn et al.: "Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12." in: Infection and immunity, Vol. 55, Issue 6, pp. 1466-75, 1987 (PubMed).

Thole, Dauwerse, Das et al.: "Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli." in: Infection and immunity, Vol. 50, Issue 3, pp. 800-6, 1986 (PubMed).