Bacillus Anthracis Lethal Factor (LF) Antikörper

Antigen: Bacillus Anthracis Lethal Factor (LF)

Klonalität: Monoklonal (5F502-2)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN121211

Produktbeschreibung

Weitere Bezeichnung: Bacillus anthracis lethal factor

Swiss-Prot: P15917

Immunogen: Synthetic peptide corresponding to amino acids 779-792 of the Anthrax LF protein (~90 kD)

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 5F502-2

Beschreibung: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family2.

Spezifität: This antibody is specific for Anthrax LF (Lethal Factor) protein.

Anwendungen

Applikationshinweise: Western blot analysis (0.1 ug/ml, positive control: recombinant protein). In Daudi, a ~70 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.5 mg/ml

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Bakterien, Gram-positive Bakterien, Toxine, Virologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of Anthrax LF in recombinant protein using ABIN121211 at 0.1 ug/ml.

Publikationen

Publikationen: Collier, Young: "Anthrax toxin." in: Annual review of cell and developmental biology, Vol. 19, pp. 45-70, 2003 (PubMed).