Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 1 (SLC8A1) Antikörper

Antigen: Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 1 (SLC8A1)

Synonyme: NCX1, FLJ37694, FLJ43417, MGC119581, DKFZp779F0871, Ncx1, AI852629, AV344025, MGC90693, D930008O12Rik, Ncx, SLC8A1, ncx1n, DKFZp469I106

Klonalität: Monoklonal (C2C12)

Wirt: Maus

Reaktivität: Hund, Meerschweinchen, Human, Kaninchen, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN121034

Produktbeschreibung

Weitere Bezeichnung: SLC8A1 / NCX1

Swiss-Prot: P32418

Immunogen: Purified canine cardiac sodium/calcium exchanger

Format: Ascites

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: C2C12

Beschreibung: The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells. Alternate Names: Na-Exchange Protein 1, SLC8A1, CNC, NaCX-1.

Spezifität: This antibody detects sodium/calcium exchanger from Human, Canine, Rabbit, Guinea Pig and Rat kidney and cardiac tissues. It recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.

Anwendungen

Applikationshinweise: Immunofluorescence (1/200): results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane in rat heart. Immunoprecipitation. Western Blot (1/1,000): detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. ABIN121034 is not recommended for Western blot procedures of rat tissues. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: This antibody is supplied as liquid, PBS-diluted ascites containing 0.05% sodium azide as preservative.

Lagerung: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar