Sodium/potassium ATPase beta Subunit Antikörper

Antigen: Sodium/potassium ATPase beta Subunit

Synonyme: Atpb, Atpb-1

Klonalität: Monoklonal (2G11)

Wirt: Maus

Reaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN121031

Produktbeschreibung

Weitere Bezeichnung: ATP4B

Swiss-Prot: P51164

Immunogen: Purified 34 kDa core peptide from deglycosylated hog gastric microsomes

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 2G11

Beschreibung: The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation- transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

Spezifität: This antibody detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues. This antibody has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with MA3-923 results in specific staining of parietal cells. MA3-923 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site. The MA3-923 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.

Anwendungen

Applikationshinweise: Western Blot: 1:4,000. Immunoprecipitation: Assay dependent. Immunohistochemistry on paraffin sections: 1:2,000. Immunofluorescence: 1:2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.05% sodium azide

Lagerung: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar