Calmodulin (CALM) Antikörper

Antigen: Calmodulin (CALM)

Synonyme: 3909, CAM, Cal, Cal49A, D-CaM, DmCalm, anon-EST:Posey59, dCaM, l(2)03909, l(2)3909, DmelCG8472, CG8472, calm2, cam, calm1, calmodulin, Cam, ACYPI000056, CALM3, CaM, CALM, Calmodulin, DKFZp469E1312, LOC100194628, PHKD, CAMII, PHKD2, FLJ99410, PHKD3, Calm, AI256814, AI327027, AI461935, AL024000, CaMI, CaMII

Klonalität: Monoklonal (6D4)

Wirt: Maus

Reaktivität: Rind (Kuh), Huhn, Ratte (Rattus)

Applikation: Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN121026

Produktbeschreibung

Weitere Bezeichnung: Calmodulin

Swiss-Prot: P02599

Immunogen: Calmodulin purified from Dictyostelium discoideum

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 6D4

Beschreibung: Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic Western blot under reducing conditions (3 µg/lane Apolipoprotein E), this antibody gave a major band at ~30 kDa when used at 1/500-1/1000., cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Spezifität: ABIN121026 recognizes calmodulin . This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

Synonyme: 3909, CAM, Cal, Cal49A, D-CaM, DmCalm, anon-EST:Posey59, dCaM, l(2)03909, l(2)3909, DmelCG8472, CG8472, calm2, cam, calm1, calmodulin, Cam, ACYPI000056, CALM3, CaM, CALM, Calmodulin, DKFZp469E1312, LOC100194628, PHKD, CAMII, PHKD2, FLJ99410, PHKD3, Calm, AI256814, AI327027, AI461935, AL024000, CaMI, CaMII

Anwendungen

Applikationshinweise: ELISA. Immunocytochemistry (1/100) results in staining of the contractile vacuoles in Dictyostelium cells. Western Blot (1/2000) detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.05% sodium azide as preservative.

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: One year from despatch.

Forschungsgebiet: Krebs, Signaltransduktion, Hormone, Zytokine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 2 shows a Western blot of purified calmodulin using SM5111 and ABIN121026.