Prion Protein (PRNP) Antikörper

Antigen: Prion Protein (PRNP)

Synonyme: CJD, GSS, PrP, ASCR, PRIP, PrPc, CD230, prion, MGC26679, PrP27-30, PrP33-35C, Prn, MGC140197, prp, Prn-p, cjd, prp(c), MGC84114, PRNP, PRP, Prp, SIP, PRPC, DKFZp459A016

Klonalität: Monoklonal (F89-160-1-5)

Wirt: Maus

Reaktivität: Rind (Kuh), Human, Schaf

Applikation: Enzyme Immunoassay (EIA), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN120959

Produktbeschreibung

Weitere Bezeichnung: CD230 / PrP

Swiss-Prot: P04156

Immunogen: Synthetic Peptide: S(146) R P L I H F G S D Y E D R(159) corresponding to amino acid residues 146-159 of bovine PrP.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: F89-160-1-5

Beschreibung: Cellular prion protein (PrPC) is a highly conserved, 33-35 kDa protease-sensitive, sialoglycoprotein of unknown function which is endogenously expressed in brain. Studies have shown this protein to be anchored to the external surface of the cell membrane by glycosyl phosphatidylinositol. A post-translationally altered, protease-resistant PrPC isoform ( PrP- Scrapie or PrPSc) of 27-30 kDa is found in association with several fatal neurodegenerative disorders collectively termed transmissible spongiform encephalopathies (TSEs). Deposition of PrPSc in the central nervous system is a reliable indication of a TSE. Prion diseases include scrapie in sheep and goats, bovine spongiform encephalopathy or Mad Cow disease, and chronic wasting disease (CWD) in deer and elk. PrP is responsible for Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler-Scheinker (GSS) disease, Fatal Familial Insomnia (FFI), and Kuru in humans.

Spezifität: This antibody detects prion protein (PrP) protein from a variety of species that have the conserved n-IHFG-c epitope, including agriculturally important animal species such as sheep, bovine, deer, and elk. ABIN120959 is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c. This peptide (ABIN126319) is available for use in Neutralization and Control experiments

Anwendungen

Applikationshinweise: Immunohistochemistry on paraffin embedded sections: 3-5 ug/ml, staining of PrP(Sc) in infected sheep brain with ABIN120959 results in intense staining of the spongiform lesions. This antibody detects only the PrP(Sc) and not PrP(C). Western Blot: 3-5 ug/ml, detects a 33-35 kDa protein from normal animals and a 27-30 kDa protein which represents PrP in brain protease treated tissue extracts from infected animals. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative.

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar