Vitamin D3 Receptor (VDR) Antikörper

Antigen: Vitamin D3 Receptor (VDR)

Synonyme: NR1I1, Nr1i1, VDR, LOC100008907, LOC100136371

Klonalität: Monoklonal (9A7)

Wirt: Ratte

Reaktivität: Amphibien, Huhn, Fisch, Hamster, Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Elektromobilitäts-Experimente (EMSA), Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB), Chromatin-Immunopräzipitation (ChiP)

Produktnummer: ABIN120949

Produktbeschreibung

Weitere Bezeichnung: Vitamin D3 receptor / NR1I1

Swiss-Prot: P11473

Immunogen: Partially purified chicken intestinal cytoplasmic 1,25-(OH)2D3 receptor protein

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: 9A7

Beschreibung: Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T- cell proliferation and interleukin-2 (IL-2) gene expresion, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription.

Spezifität: ABIN120949 detects vitamin D receptor (VDR), it recognizes an epitope between amino acid residues 89 and 105 of human VDR. This region is just C-terminal to the DNA binding domain of the VDR. This antibody does not cross-react with estrogen or glucocorticoid receptors. ABIN120949 detects both the occu pied and unoccu pied forms of the receptor.

Anwendungen

Applikationshinweise: Gel Shift. Immunocytochemistry. Immunohistochemistry on frozen and paraffin embedded sections (5 ug/ml): results in strong nuclear staining in cultured human skin fibroblasts. Immunoprecipitation. Western Blot (1 ug/ml): detects a 58 kDa and 60 kDa protein representing VDR in avians while the VDRs of mammalian species appear as single bands within the 52-55 kDa range. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: This antibody is supplied as liquid, purified immunoglobulin fraction in PBS with 0.05% sodium azide as preservative.

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Vitamine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot of VDR in human skin fibroblast extract using ABIN120949.