Estrogen Receptor 1 (ESR1) Antikörper

Antigen: Estrogen Receptor 1 (ESR1)

Synonyme: AAC2, PNAT, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, 7B4, CD144, FLJ17376, ER, ESR, Era, ESRA, NR3A1, DKFZp686N23123, ERa, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ER[b], Estrb, ERbeta, Esr, RNESTROR, eralpha, zfER[a], ESR2, ERB, Errra, ERRalpha, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, erb, esrb, estrb, nr3a2, er-beta, esr-beta, XER, era, esr, esra, nr3a1, xesr-1, xlERalpha1, xlERalpha2, CERB, ER-beta, ESR1, LOC398733, LOC398734, ESRRA, ESTRRA, ERBETA, ERALPHA, err1, erra, esrral, erralpha, esrl1, nr3b1, MGC147343, er, LOC100125507

Klonalität: Monoklonal (33)

Wirt: Maus

Reaktivität: Rind (Kuh), Human, Maus, Ratte (Rattus)

Applikation: Elektromobilitäts-Experimente (EMSA), Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN120937

Produktbeschreibung

Weitere Bezeichnung: Estrogen receptor alpha

Swiss-Prot: P03372

Immunogen: Synthetic peptide, corresponding to amino acid residues 247-261 of the ER alpha DNA binding domain AA Sequence:E(247) V G M K G G I R K D R R G(261)

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 33

Beschreibung: The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.

Spezifität: This antibody detects both the steroid occu pied and unoccu pied forms of the estrogen receptor (ER) alpha. ABIN120937 does not cross react with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor.

Synonyme: AAC2, PNAT, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, 7B4, CD144, FLJ17376, ER, ESR, Era, ESRA, NR3A1, DKFZp686N23123, ERa, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ER[b], Estrb, ERbeta, Esr, RNESTROR, eralpha, zfER[a], ESR2, ERB, Errra, ERRalpha, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, erb, esrb, estrb, nr3a2, er-beta, esr-beta, XER, era, esr, esra, nr3a1, xesr-1, xlERalpha1, xlERalpha2, CERB, ER-beta, ESR1, LOC398733, LOC398734, ESRRA, ESTRRA, ERBETA, ERALPHA, err1, erra, esrral, erralpha, esrl1, nr3b1, MGC147343, er, LOC100125507

Anwendungen

Applikationshinweise: Flow Cytometry. Gel Shift. Immunocytochemistry (10 ug/ml). Immunohistochemistry (5 ug/ml): staining in mouse Leydig cells results in both nuclear and cytoplasmic staining). Immunoprecipitation. Western Blot (1 ug/ml): detects a ~67 kDa protein representing ER alpha from MCF-7 cell extract). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.5 mg/ml

Buffer: This antibody is supplied as liquid, ascites diluted in PBS containing 0.09% sodium azide as preservative.

Lagerung: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Hormone, Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot on recombinant ER alpha using ABIN120937.

Publikationen

Publikationen: Betz, Blood, Blood: "University students' perceptions of pre-school and kindergarten children who stutter." in: Journal of communication disorders, Vol. 41, Issue 3, pp. 259-73, 2008 (PubMed).