Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
The antibody recognizes the defined epitope (aa 196-212) of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
Aufreinigung
Protein A affinity chromatography
Reinheit
> 95 % (by SDS-PAGE)
Immunogen
Peptide corresponding to aa 196-212 of human NTAL coupled to KLH
LAT2
Reaktivität: Human
IHC (p), IF (p)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Immunoprecipitation. Western Blot: Recommended dilution: 1-2 mg/mL, incubation 60 min. Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Haltbarkeit
12 months
Target
LAT2
(Linker For Activation of T Cells Family, Member 2 (LAT2))
LAB antikoerper, NTAL antikoerper, WBSCR15 antikoerper, WBSCR5 antikoerper, WSCR5 antikoerper, LAT2 antikoerper, MGC139435 antikoerper, Ntal antikoerper, Wbscr5 antikoerper, AW125574 antikoerper, Wbscr15 antikoerper, linker for activation of T-cells family member 2 antikoerper, linker for activation of T cells family, member 2 antikoerper, LAT2 antikoerper, Lat2 antikoerper
Hintergrund
NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein