The antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Aufreinigung
Protein-A affinity chromatography
Reinheit
> 95 % pure (by SDS-PAGE)
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
Flow Cytometry. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Haltbarkeit
12 months
Target
HLA-E
(HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
EA1.2 antikoerper, EA2.1 antikoerper, HLA-6.2 antikoerper, MHC antikoerper, QA1 antikoerper, major histocompatibility complex, class I, E antikoerper, HLA-E antikoerper, PATR-E antikoerper
Hintergrund
HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.2, HLAE, MHC class I antigen E, alpha chain E