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PAG1 Antikörper (AA 97-432)

PAG1 Reaktivität: Human WB, IHC (p), FACS Wirt: Maus Monoclonal MEM-255 unconjugated
Produktnummer ABIN120652
  • Target Alle PAG1 Antikörper anzeigen
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    Bindungsspezifität
    • 16
    • 10
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 97-432
    Reaktivität
    • 28
    • 27
    • 24
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 43
    • 4
    Maus
    Klonalität
    • 43
    • 4
    Monoklonal
    Konjugat
    • 19
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PAG1 Antikörper ist unkonjugiert
    Applikation
    • 34
    • 16
    • 13
    • 13
    • 12
    • 8
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Spezifität
    The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
    Aufreinigung
    Protein-A affinity chromatography
    Reinheit
    > 95 % pure (by SDS-PAGE)
    Immunogen
    Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
    Klon
    MEM-255
    Isotyp
    IgG2a
    Top Product
    Discover our top product PAG1 Primärantikörper
  • Applikationshinweise
    Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    Andere Bezeichnung
    PAG1 / Csk-Binding Protein (PAG1 Produkte)
    Synonyme
    Cbp antikoerper, F730007C19Rik antikoerper, Pag antikoerper, CBP antikoerper, PAG antikoerper, MGC80192 antikoerper, phosphoprotein associated with glycosphingolipid microdomains 1 antikoerper, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 antikoerper, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog antikoerper, Pag1 antikoerper, PAG1 antikoerper, pag1.L antikoerper, pag1 antikoerper
    Hintergrund
    PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
    Gen-ID
    55824
    UniProt
    Q9NWQ8
    Pathways
    p53 Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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