Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) Antikörper

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Antigen
  • MADCAM1
  • AV211525
  • MACAM1
  • mucosal vascular addressin cell adhesion molecule 1
  • MADCAM1
  • Madcam1
  • MADCAM-1
Reaktivität
Human
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100
33
2
Wirt
Maus
107
61
45
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
25
12
9
7
5
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
115
112
45
43
35
29
26
23
10
7
7
3
3
2
2
2
2
2
2
1
1
1
1
Optionen
Hersteller
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Immunogen Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Klon 17F5
Isotyp IgG1
Spezifität This antibody recognizes Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). Clone 17F5 recognises the Mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1.
Reinigung Affinity Chromatography on Protein G.
Andere Bezeichnung MAdCAM1 (MADCAM1 Antibody Abstract)
Hintergrund Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1) is a 60kD transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyers patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
Gen-ID 8174
NCBI Accession NP_570116
UniProt Q13477
Applikationshinweise Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50 - 1/100, This product does not require protein digestion prior to staining, This product does not require HIER prior to staining. ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Allgemeine Veröffentlichungen Leung, Lehnert, Kanwar, Yang, Mon, McNeil, Krissansen: "Bioassay detects soluble MAdCAM-1 in body fluids." in: Immunology and cell biology, Vol. 82, Issue 4, pp. 400-9, 2004 (PubMed).

Leung, Greene, Ni, Raymond, Lehnert, Langley, Krissansen: "Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts." in: Immunology and cell biology, Vol. 74, Issue 6, pp. 490-6, 1997 (PubMed).

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