Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) Antikörper

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  • MADCAM1
  • AV211525
  • MACAM1
  • mucosal vascular addressin cell adhesion molecule 1
  • MADCAM1
  • Madcam1
  • MADCAM-1
Reaktivität
Human
129
100
33
2
Wirt
Maus
105
61
45
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
27
14
8
6
6
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
116
110
45
43
36
29
26
23
10
9
7
3
2
2
2
2
2
2
1
1
1
1
1
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Immunogen Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Klon 314G8
Isotyp IgG1
Spezifität This antibody recognises Human mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.
Reinigung Affinity Chromatography on Protein G
Endotoxin-Niveau Low Endotoxin
Andere Bezeichnung MAdCAM1 (MADCAM1 Antibody Abstract)
Hintergrund MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
Gen-ID 8174
NCBI Accession NP_570116
UniProt Q13477
Applikationshinweise ELISA. Western Blot: this antibody detects a band of approximately 60 kDa. Functional Assays. Flow Cytometry: 1/10 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining, This product does not require antigen retrieval prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives or stabilizers
Konservierungsmittel Without preservative
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Allgemeine Veröffentlichungen Olloquequi, Montes, Prats, Rodríguez, Montero, García-Valero, Ferrer: "Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients." in: The European respiratory journal, Vol. 37, Issue 2, pp. 289-98, 2011 (PubMed).

Leung, Lehnert, Kanwar, Yang, Mon, McNeil, Krissansen: "Bioassay detects soluble MAdCAM-1 in body fluids." in: Immunology and cell biology, Vol. 82, Issue 4, pp. 400-9, 2004 (PubMed).

Leung, Greene, Ni, Raymond, Lehnert, Langley, Krissansen: "Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts." in: Immunology and cell biology, Vol. 74, Issue 6, pp. 490-6, 1997 (PubMed).