TUBA1B Antikörper
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- Target Alle TUBA1B Antikörper anzeigen
- TUBA1B (Tubulin, alpha 1B (TUBA1B))
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Reaktivität
- Avian, Säugetier, Saccharomyces cerevisiae
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Wirt
- Ratte
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Klonalität
- Monoklonal
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Konjugat
- Dieser TUBA1B Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- Spezifität
- SM2202P recognises the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).
- Aufreinigung
- Affinity Chromatography on Protein G
- Immunogen
- Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
- Klon
- YL1-2
- Isotyp
- IgG2a
- Top Product
- Discover our top product TUBA1B Primärantikörper
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- Applikationshinweise
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Immunohistochemistry on frozen sections. ELISA (1/100-1/1000, routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- TUBA1B (Tubulin, alpha 1B (TUBA1B))
- Andere Bezeichnung
- alpha Tubulin / TUBA1B (TUBA1B Produkte)
- Synonyme
- RGD1565476 antikoerper, K-ALPHA-1 antikoerper, cb944 antikoerper, fb22g06 antikoerper, tuba1 antikoerper, wu:fb22g06 antikoerper, Tuba2 antikoerper, TUBA3 antikoerper, k-alpha-1 antikoerper, tuba antikoerper, Calpha1 antikoerper, Tuba1 antikoerper, TUBA1B antikoerper, tubulin, alpha 1B antikoerper, tubulin alpha 1b antikoerper, tubulin, alpha 1b antikoerper, tubulin alpha 1b L homeolog antikoerper, tubulin alpha-1B chain antikoerper, Tubulin alpha-1B chain antikoerper, Tuba1b antikoerper, TUBA1B antikoerper, tuba1b antikoerper, tuba1b.L antikoerper, LOC100445003 antikoerper, LOC102178700 antikoerper
- Hintergrund
- Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain
- Gen-ID
- 10376
- NCBI Accession
- NP_006073
- UniProt
- P68363
- Pathways
- Microtubule Dynamics, M Phase
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