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S100A8/A9 Complex (Calprotectin) Antikörper

S100A8/A9 Reaktivität: Human, Rind (Kuh), Schwein, Meerschweinchen, Pferd, Affe, Kaninchen, Ratte, Cat, Hund FACS, IHC (p), IHC (fro) Wirt: Maus Monoclonal MAC387 unconjugated
Produktnummer ABIN119774
  • Target Alle S100A8/A9 Complex (Calprotectin) (S100A8/A9) Antikörper anzeigen
    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
    Reaktivität
    • 33
    • 13
    • 13
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 9
    • 9
    • 9
    Human, Rind (Kuh), Schwein, Meerschweinchen, Pferd, Affe, Kaninchen, Ratte, Cat, Hund
    Wirt
    • 15
    • 15
    • 3
    Maus
    Klonalität
    • 18
    • 15
    Monoklonal
    Konjugat
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist unkonjugiert
    Applikation
    • 15
    • 13
    • 12
    • 8
    • 7
    • 5
    • 4
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 12 kDa beta chain expressed by Granulocytes, Monocytes and by tissue Macrophages. Variable results have been reported for staining brain macrophages and microglia.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human, Horse, Porcine, Canine, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Feline, Cynomologus monkey, Rhesus monkey and Goat.
    Aufreinigung
    Affinity Chromatography on Protein G
    Immunogen
    Human Monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    Klon
    MAC387
    Isotyp
    IgG1
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    Discover our top product S100A8/A9 Primärantikörper
  • Applikationshinweise
    Immunohistochemistry on Frozen sections (1/100-1/200). Immunohistochemistry on Paraffin Embedded Sections (1/100-1/200): requires protein digestion pre-treatment e.g. trypsin, 0.1 % for 10 minutes or antigen retrieval using heat treatment prior to staining, see also Ref.2. Recommended Positive Control Tissue: Human Spleen. Flow Cytometry (1/50-1/100): membrane permeabilisation required, see also Ref.1.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
    Andere Bezeichnung
    MRP8 / MRP14 (S100A8/A9 Produkte)
    Synonyme
    60B8AG antikoerper, CAGB antikoerper, CFAG antikoerper, CGLB antikoerper, L1AG antikoerper, LIAG antikoerper, MAC387 antikoerper, MIF antikoerper, MRP14 antikoerper, NIF antikoerper, P14 antikoerper, S100 calcium binding protein A9 antikoerper, S100A9 antikoerper
    Hintergrund
    Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).Synonyms: MRP8/14, S100A8, S100A8/A9, S100A9
    Pathways
    S100 Proteine
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