Junctional Adhesion Molecule 2 (JAM2) Antikörper

Antigen: Junctional Adhesion Molecule 2 (JAM2)

Synonyme: JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Klonalität: Monoklonal (CRAM-18 F26)

Wirt: Ratte

Reaktivität: Human, Maus

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS)

Produktnummer: ABIN119734

Produktbeschreibung

Weitere Bezeichnung: CD322 / JAM2

Swiss-Prot: P57087

Immunogen: Recombinant soluble JAM-2

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: CRAM-18 F26

Beschreibung: JAM-2, a 45kD transmembrane type 1 protein, a member of the immunoglobulin superfamily which is also known as CD322. Alternate Names: JAM-B, JAMB, JAM-2, VE-JAM, VEJAM, C21orf43, Junctional Adhesion Molecule 2, Junctional Adhesion Molecule B, Vascular Endothelial Junction-Associated Molecule.

Spezifität: Recognises Mouse junctional adhesion molecule-2 (JAM-2, CD322). In adult murine tissue, CD322 expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, CD322 expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Synonyme: JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Anwendungen

Applikationshinweise: Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 ul of 1/10-1/25 diluted antibody to label 10e6 cells in 100 ul. Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (2). We recommend ABIN119731 for this purpose. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml IgG

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Zell-/Gewebemarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Aurrand-Lions, Duncan, Ballestrem et al.: "JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2733-41, 2001 (PubMed).

Aurrand-Lions, Johnson-Leger, Wong et al.: "Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members." in: Blood, Vol. 98, Issue 13, pp. 3699-707, 2001 (PubMed).