His Tag Antikörper

Details zu Produkt Nr. ABIN119353, Anbieter: Anmelden zum Anzeigen
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reaktivität
Tag
616
24
22
14
3
2
Wirt
Maus
434
184
27
23
4
4
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
554
386
166
107
99
77
69
25
13
10
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen PAX6 transcription factor linked to histidine tag. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Sequenz HHHHHH
Klon AD1-1-10
Isotyp IgG1
Reinigung Affinity Chromatography on Protein G
Andere Bezeichnung 6xHistidine Epitope Tag
Substanzklasse Tag
Hintergrund The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.
Applikationshinweise Immunhistology on Frozen Sections. ELISA. Immunoprecipitation. Western Blot (1/500-1/1000). In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (ABIN119353) Western blot of HIS-tagged protein probed with mouse anti histidine tag (ABIN119353) ...
Allgemeine Veröffentlichungen De Vooght, Caljon, Stijlemans, De Baetselier, Coosemans, Van den Abbeele: "Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly." in: Microbial cell factories, Vol. 11, pp. 23, 2012 (PubMed).

Diefenbacher, Thorsteinsdottir, Spang: "The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 25027-38, 2011 (PubMed).

Alvarez, López-Pacheco, Aguilar-Yañez, Portillo-Lara, Mendoza-Ochoa, García-Echauri, Freiden, Schultz-Cherry, Zertuche-Guerra, Bulnes-Abundis, Salgado-Gallegos, Elizondo-Montemayor, Hernández-Torre: "Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method." in: PLoS ONE, Vol. 5, Issue 4, pp. e10176, 2010 (PubMed).

Gunnarsen, Lunde, Kristiansen, Bogen, Sandlie, Løset: "Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA." in: BMC biotechnology, Vol. 10, pp. 8, 2010 (PubMed).

Wrighton, Lin, Yu, Feng: "Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9755-63, 2009 (PubMed).

Bahi, Dreyer: "Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization." in: Genes, brain, and behavior, Vol. 7, Issue 2, pp. 244-56, 2008 (PubMed).

Hwang, Duvall, Tomlinson, Boackle et al.: "Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable ..." in: Molecular immunology, Vol. 45, Issue 9, pp. 2570-80, 2008 (PubMed).

Bahi, Kusnecov, Dreyer: "The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 33, Issue 11, pp. 2726-34, 2008 (PubMed).

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008 (PubMed).

Hoffmann, Schellack, Textor, Konold, Schmitz, Cerwenka, Pflanz, Watzl: "Identification of CLEC12B, an inhibitory receptor on myeloid cells." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22370-5, 2007 (PubMed).

Zheng, Kohler, Chen, Weber, Khan, Johnson, Orentas: "Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen." in: BMC immunology, Vol. 8, pp. 4, 2007 (PubMed).

Bahi, Boyer, Kafri, Dreyer: "CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus." in: The European journal of neuroscience, Vol. 19, Issue 6, pp. 1621-33, 2004 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

Suen, Wu, Chen, Wu, Chiang: "Characterization of self-T-cell response and antigenic determinants of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice." in: Immunology, Vol. 103, Issue 3, pp. 301-9, 2001 (PubMed).

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