The antibody reacted with denaturated HLA-G heavy chain. The recognized epitope is present only on HLA-G1, -G2 and -G5 Molecules. HLA-G1 and -G2 are membrane bound proteins, HLA-G5 is a soluble protein.
Aufreinigung
Protein A affinity purified immunoglobulin fraction
Western Blotting (MWs: HLA-G1 approx. 45 kDa, HLA-G2 approx. 45 kDa, HLA-G5 approx. 28 kDa). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline pH 7.4 with 15 mM sodium azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Haltbarkeit
6 months
Target
HLAG
(HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
MHC-G antikoerper, B-F antikoerper, B-F-S04 antikoerper, B-F-S05 antikoerper, B-F-S06 antikoerper, B-F-S07 antikoerper, B-FI antikoerper, B-FIV antikoerper, BF2 antikoerper, BFa2 antikoerper, BFw-03 antikoerper, BFw-05 antikoerper, BFz-01 antikoerper, major histocompatibility complex, class I, G antikoerper, MHC BF1 class I antikoerper, HLA-G antikoerper, BF1 antikoerper
Hintergrund
HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G