ITGA2 Antikörper

Produktnummer ABIN118720

Produktdetails anti-ITGA2 Antikörper

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGA2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Purified human beta1 preparation from HT1080 fibrosarcoma cell extract. Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.

Klon: 16B4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 30 μg/mL, Immunoprecipitation: 25-50 μg/mL. Immunohistochemistry on Frozen Sections: 30-40 μg/mL. ELISA: 10 μg/mL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Andere Bezeichnung: CD49b / ITGA2 (ITGA2 Produkte)

Synonyme: BR antikoerper, CD49B antikoerper, GPIa antikoerper, HPA-5 antikoerper, VLA-2 antikoerper, VLAA2 antikoerper, DX5 antikoerper, integrin subunit alpha 2 antikoerper, integrin alpha 2 antikoerper, ITGA2 antikoerper, Itga2 antikoerper

Hintergrund: CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2

Gen-ID: 3673

NCBI Accession: NP_002194

UniProt: P17301

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex