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Sialoadhesin/CD169 Antikörper

SIGLEC1 Reaktivität: Maus FACS, IHC (fro) Wirt: Ratte Monoclonal MOMA-1 unconjugated
Produktnummer ABIN118292
  • Target Alle Sialoadhesin/CD169 (SIGLEC1) Antikörper anzeigen
    Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
    Reaktivität
    • 48
    • 23
    • 20
    • 4
    • 3
    Maus
    Wirt
    • 36
    • 19
    • 18
    • 4
    Ratte
    Klonalität
    • 38
    • 36
    • 1
    Monoklonal
    Konjugat
    • 32
    • 9
    • 9
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Sialoadhesin/CD169 Antikörper ist unkonjugiert
    Applikation
    • 46
    • 30
    • 23
    • 20
    • 15
    • 13
    • 13
    • 13
    • 10
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    Clone MOMA-1 is a useful marker for the identification of Macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone Macrophages as detected by ER-TR9 (Cat.-No BM4011). In addition , MOMA-1 detects Macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived Macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF). The antigen detected by MOMA-1 is CD169, also known as Sialoadhesin. Antigen Distribution Isolated Cells: No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident Macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue Sections: Distinct Macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a Macrophage subpopulation in the lamina propria of the villi of the small intestine.Antigen Distribution
    Kreuzreaktivität (Details)
    Species reactivity (tested):Mouse: Subpopulation of Mature Resident Tissue Macrophages.
    Aufreinigung
    Affinity Chromatography.
    Immunogen
    Mouse lymph node tissue. Remarks: The antigen is found intracellularly and on cell surface on Metallophilic Macrophages.
    Klon
    MOMA-1
    Isotyp
    IgG2a
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  • Applikationshinweise
    Immunohistochemistry on frozen sections: 2 μg/mL (1/200). Fixation: Acetone, Positive control: Mouse spleen. Flow cytometry. Does not react on routinely processed paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).
    Konzentration
    0.4 mg/mL
    Buffer
    Stock solutions contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20 °C upon receipt.
  • Target
    Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
    Andere Bezeichnung
    CD169 / SIGLEC1 (SIGLEC1 Produkte)
    Synonyme
    Cd169 antikoerper, Siglec-1 antikoerper, Sn antikoerper, CD169 antikoerper, SIGLEC-1 antikoerper, SN antikoerper, dJ1009E24.1 antikoerper, SA antikoerper, SIGLEC1 antikoerper, p210 antikoerper, pSn antikoerper, sialic acid binding Ig like lectin 1 antikoerper, sialic acid binding Ig-like lectin 1, sialoadhesin antikoerper, SIGLEC1 antikoerper, Siglec1 antikoerper
    Hintergrund
    Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1
    Gen-ID
    20612
    NCBI Accession
    NP_035556
    UniProt
    Q62230
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