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CD40 Antikörper (CD40 Molecule, TNF Receptor Superfamily Member 5) (Extracellular Domain) (FITC)

Details für Produkt anti-CD40 Antikörper Nr. ABIN118120, Anbieter: Anmelden zum Anzeigen
Antigen
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Alternativen
anti-Maus CD40 Antikörper für Flow Cytometry
Epitop
Extracellular Domain
55
39
36
25
22
19
17
11
8
7
5
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Maus
637
314
67
21
15
12
11
8
4
3
2
2
1
1
1
1
Wirt
Ratte
502
213
161
23
4
4
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD40 Antikörper ist konjugiert mit FITC
94
93
53
23
21
18
15
13
13
12
12
9
9
9
9
9
9
9
8
7
6
6
6
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
575
235
204
153
105
103
95
63
46
33
19
13
13
9
8
8
6
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Bilder

Immunogen Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Klon 01-03-23
Isotyp IgG2a
Spezifität Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Reinigung Affinity Chromatography on Protein G
Andere Bezeichnung CD40 (CD40 Antibody Abstract)
Hintergrund CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gen-ID 21939
NCBI Accession NP_035741
UniProt P27512
Forschungsgebiet Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin
Applikationshinweise Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing lowaffinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD40 Antikörper (CD40 Molecule, TNF Receptor Superfamily Member 5) (Extracellular Domain) (FITC) (ABIN118120) Staining of mouse spleen cells with RAT ANTI MOUSE CD40:FITC
Allgemeine Veröffentlichungen Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994 (PubMed).

Parry, Holman, Hasbold, Klaus: "Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes." in: European journal of immunology, Vol. 24, Issue 4, pp. 974-9, 1994 (PubMed).

Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994 (PubMed).