SKP1 Antikörper (C-Term)

Produktnummer ABIN117959

Produktdetails anti-SKP1 Antikörper

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Bindungsspezifität: AA 152-163, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequenz: Human SKP1 (163 aa 18.5 kDa) 1 mpsiklqssd geifevdvei akqsvtiktm ledlgmddeg dddpvplpnv naailkkviq 61 wcthhkddpp ppeddenkek rtddipvwdq eflkvdqgtl felilaanyl dikglldvtc 121 ktvanmikgk tpeeirktfn ikndfteeee aqvrkenqwc eek

Spezifität: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.

Aufreinigung: Delipidation and defibrination.

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.

Anwendungsinformationen

Applikationshinweise: ELISA (1/2,000-1/10,000). This antibody reacts with human SKP1 by Western blot(1/500-1/1,000) and Immunoprecipitation. The antibody Immunoprecipitates in vitrotranslated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band correspondingto human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positivecontrol.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85.0 mg/mL (by Refractometry)

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Referenzen für anti-SKP1 Antikörper (ABIN117959)

Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, (2008) (PubMed).

Details zu SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Andere Bezeichnung: SKP1 / OCP2 (SKP1 Produkte)

Synonyme: EMC19 antikoerper, OCP-II antikoerper, OCP2 antikoerper, SKP1A antikoerper, TCEB1L antikoerper, p19A antikoerper, 15kDa antikoerper, 2610043E24Rik antikoerper, 2610206H23Rik antikoerper, SKP1 antikoerper, Tceb1l antikoerper, p19Skp1 antikoerper, Skp1a antikoerper, skp1a antikoerper, zgc:73186 antikoerper, ocp2 antikoerper, emc19 antikoerper, ocp-ii antikoerper, tceb1l antikoerper, ARABIDOPSIS SKP1 HOMOLOGUE 1 antikoerper, ASK1 antikoerper, ATSKP1 antikoerper, S phase kinase-associated protein 1 antikoerper, T4O12.17 antikoerper, T4O12_17 antikoerper, UFO INTERACTING PROTEIN 1 antikoerper, UIP1 antikoerper, ocp-2 antikoerper, S-phase kinase associated protein 1 antikoerper, S-phase kinase-associated protein 1 antikoerper, S-phase kinase-associated protein 1 pseudogene antikoerper, S-phase kinase-associated protein 1A antikoerper, S-phase kinase-associated protein 1 S homeolog antikoerper, S phase kinase-associated protein 1 antikoerper, SKP1 antikoerper, skp1 antikoerper, LOC100340043 antikoerper, Skp1a antikoerper, Skp1 antikoerper, skp1.S antikoerper

Hintergrund: SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X.Synonyms: Cyclin A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, Organ of Corti protein 2, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SKP1A, TCEB1L, Transcription elongation factor B, p19A, p19skp1

Gen-ID: 6500

NCBI Accession: NP_008861

UniProt: P63208

Pathways: Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases