MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457)

Details for Product anti-FRAP1 Antibody No. ABIN117909, Anbieter: Anmelden zum Anzeigen
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternativen
anti-Human MTOR Antikörper für Chromatin Immunoprecipitation
Epitop
AA 2440-2457, pSer2448
63
28
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15
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1
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Reaktivität
Human
368
201
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18
9
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4
2
2
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Wirt
Kaninchen
316
48
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Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
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Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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37
20
7
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3
2
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2
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Optionen
Hersteller
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Immunogen A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR
Spezifität Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies.
Kreuzreaktivität (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Reinigung Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Andere Bezeichnung mTOR / FRAP1 (FRAP1 Antibody Abstract)
Hintergrund Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gen-ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Applikationshinweise This antibody has been tested for use in Immunohistochemistry (5.0 μg/mL), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.88 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Bilder des Herstellers
 image for anti-MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN117909) Immunoblotting. Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250...
 image for anti-MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN117909) Immunoblotting. Metabolic and autocrine regulation of the mTOR pathway by beta-cells.
Allgemeine Veröffentlichungen Chase, Rao, Hofmann, Chang-Hasnain: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010 (PubMed).

Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003 (PubMed).

Chen, Zheng, Foster: "Phospholipase D confers rapamycin resistance in human breast cancer cells." in: Oncogene, Vol. 22, Issue 25, pp. 3937-42, 2003 (PubMed).

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003 (PubMed).

McMahon, Choi, Lin, Abraham, Lawrence: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002 (PubMed).

Hudson, Liu, Chiang, Otterness, Loomis, Kaper, Giaccia, Abraham: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002 (PubMed).

Choi, Bertram, Drenan, Carvalho, Zhou, Zheng: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002 (PubMed).

Inoki, Li, Zhu, Wu, Guan: "TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling." in: Nature cell biology, Vol. 4, Issue 9, pp. 648-57, 2002 (PubMed).

Zhang, Shu, Hosoi, Murti, Houghton: "Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28127-34, 2002 (PubMed).