IL-10RA Antikörper

Produktnummer ABIN1177268

Produktdetails anti-IL-10RA Antikörper

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL-10RA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Neutralization (Neut)

Marke: BD Pharmingen™

Produktmerkmale: Neutralization Activity: This antibody has been reported to be useful for the neutralization of the activity elicited by recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay with 1x10^5 cells/mL D36 indicator cells using 6 ng/mL recombinant mouse IL-10 (Cat. No. 550070) costimulated with 50 pg/mL recombinant mouse IL-4 (Cat. No. 550067) (i.e preincubatiion of the antibody with the cells for 60 min)
50% Neutralization (ND50) at 0.1 - 1 ng/mL
>95% Neutralization at ≥ 1 - 15 ng/mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Purified recombinant ligand-binding domain of mIL-10R

Klon: 1B1-3a

Isotyp: IgG1 kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-IL-10RA Antikörper (ABIN1177268)

OFarrell, Liu, Moore, Mui: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).

Schlaak, Schmitt, Hüls, Meyer zum Büschenfelde, Fleischer: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).

Details zu IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Andere Bezeichnung: CD210 (IL10RA Produkte)

Synonyme: IL10R1 antikoerper, IL10RA antikoerper, CD210 antikoerper, CD210a antikoerper, CDW210A antikoerper, HIL-10R antikoerper, IL-10R1 antikoerper, IL10R antikoerper, AW553859 antikoerper, CDw210 antikoerper, CDw210a antikoerper, Il10r antikoerper, mIL-10R antikoerper, interleukin 10 receptor subunit alpha antikoerper, interleukin 10 receptor, alpha antikoerper, IL10RA antikoerper, Il10ra antikoerper

Hintergrund: The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R.
Synonyms: IL-10 Receptor

Pathways: Growth Factor Binding