STAT5A Antikörper (pTyr694) (PE-CF594)
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- Target Alle STAT5A Antikörper anzeigen
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
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Bindungsspezifität
- pTyr694
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser STAT5A Antikörper ist konjugiert mit PE-CF594
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Applikation
- Intracellular Staining (ICS)
- Marke
- BD Phosflow™
- Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Phosphorylated Sheep Stat5 Peptide
- Klon
- 47-Stat5
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product STAT5A Primärantikörper
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- Applikationshinweise
- This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I).
- Kommentare
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Signal transducer, activator of transcription 5, MGF, MPF
- Probenmenge
- 5 μL
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
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Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, (2004) (PubMed).
: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).
: "JAK/STAT signaling by cytokine receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, (1998) (PubMed).
: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).
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Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, (2004) (PubMed).
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- Target
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
- Andere Bezeichnung
- Stat5 (STAT5A Produkte)
- Synonyme
- MGF antikoerper, STAT5 antikoerper, AA959963 antikoerper, Stat5 antikoerper, STAT5A/MGF antikoerper, STATA5 antikoerper, STAT5A antikoerper, stat5 antikoerper, stat5b antikoerper, signal transducer and activator of transcription 5A antikoerper, signal transducer and activator of transcription 5a antikoerper, STAT5A antikoerper, Stat5a antikoerper, stat5a antikoerper, LOC100348518 antikoerper, LOC100716811 antikoerper
- Hintergrund
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Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699. This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
Synonyms: Signal transducer and activator of transcription 5, MGF, MPF - Pathways
- JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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