STAT6 Antikörper (pTyr641) (Alexa Fluor 647)

Produktnummer ABIN1177230

Produktdetails anti-STAT6 Antikörper (Alexa Fluor 647)

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Bindungsspezifität: pTyr641

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STAT6 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Mouse STAT6 (Y641) Peptide

Klon: J71-773-58-11

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Note: For flow cytometric analysis of human cells, Alexa Fluor® 647 Mouse Anti-Hunan Stat6 (pY641) mAb (clone 18) (Cat. No. 612601) is recommended.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen für anti-STAT6 Antikörper (Alexa Fluor 647) (ABIN1177230)

Daley, Brancato, Thomay, Reichner, Albina: "The phenotype of murine wound macrophages." in: Journal of leukocyte biology, Vol. 87, Issue 1, pp. 59-67, (2010) (PubMed).

Hale, Krutzik, Samra, Crane, Nolan: "Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus." in: PLoS ONE, Vol. 4, Issue 8, pp. e6756, (2009) (PubMed).

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, (1996) (PubMed).

Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, (1995) (PubMed).

Details zu STAT6

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Andere Bezeichnung: Stat6 (STAT6 Produkte)

Synonyme: D12S1644 antikoerper, IL-4-STAT antikoerper, STAT6B antikoerper, STAT6C antikoerper, signal transducer and activator of transcription 6 antikoerper, signal transducer and activator of transcription 6-like antikoerper, STAT6 antikoerper, Stat6 antikoerper, LOC100859543 antikoerper

Hintergrund: STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL) 2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation. Active STATs then move to the nucleus to promote transcription of cytokine-inducible genes. Seven STAT proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat6 plays an important role in signaling pathways that lead to the differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors. Moreover, IL-4, secreted by activated T lymphocytes, basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. The J71-773.58.11 antibody recognizes mouse Stat6 phosphorylated at Y641.

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response