p38 Antikörper (MAP kinase p38) (pThr180) (Pacific Blue)

Details for Product anti-p38 Antibody No. ABIN1177120, Anbieter: Anmelden zum Anzeigen
Antigen
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • mitogen-activated protein kinase 14
  • MAPK14
Epitop
pThr180
67
57
31
15
15
10
10
6
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
280
222
216
38
22
22
21
20
19
18
18
18
15
15
12
4
1
1
Wirt
Maus
198
84
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser p38 Antikörper ist konjugiert mit Pacific Blue
7
7
7
7
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Applikation
Intracellular Staining (ICS)
224
161
110
69
61
56
48
28
27
18
13
9
6
6
2
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Marke BD Phosflow™
Immunogen Phosphorylated Human p38 MAPK (pT180/pY182) Peptide
Klon 36-p38
Isotyp IgG1, kappa
Reinigung Purified from tissue culture supernatant or ascites by affinity chromatography
Andere Bezeichnung p38 MAPK (p38 Antibody Abstract)
Hintergrund Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the Mitogen Activated Protein Kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38alpha (MAPK14), beta (MAPK11), gamma (MAPK12), and delta (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP Kinase Kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways. The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38alpha, beta, gamma, and delta.
Synonyms: MK14, 11, 12, 13, CSBP1, SAPK2, 2A, 3, 4, MX12, ERK-6, ERK5
Forschungsgebiet Kinases/Phosphatases
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling
Applikationshinweise This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody is conjugated to Pacific Blue™ under optimum conditions, and unreacted Pacific Blue™ was removed.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-MAP kinase p38 (p38) (pThr180) antibody (Pacific Blue) (ABIN1177120) anti-MAP kinase p38 (p38) (pThr180) antibody (Pacific Blue)
Produkt verwendet in: Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997 (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996 (PubMed).

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).

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