FASL Antikörper

Produktnummer ABIN1177002

Produktdetails anti-FASL Antikörper

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FASL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse T lymphoma cells (L5178Y) expressing human FasL

Klon: NOK-1

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-FASL Antikörper (ABIN1177002)

Orlinick, Elkon, Chao: "Separate domains of the human fas ligand dictate self-association and receptor binding." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32221-9, (1998) (PubMed).

Oyaizu, Adachi, Hashimoto, McCloskey, Hosaka, Kayagaki, Yagita, Pahwa: "Monocytes express Fas ligand upon CD4 cross-linking and induce CD4+ T cells apoptosis: a possible mechanism of bystander cell death in HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 5, pp. 2456-63, (1997) (PubMed).

Sieg, Smith, Yildirim, Kaplan: "Fas ligand deficiency in HIV disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5860-5, (1997) (PubMed).

Villunger, Egle, Marschitz, Kos, Böck, Ludwig, Geley, Kofler, Greil: "Constitutive expression of Fas (Apo-1/CD95) ligand on multiple myeloma cells: a potential mechanism of tumor-induced suppression of immune surveillance." in: Blood, Vol. 90, Issue 1, pp. 12-20, (1997) (PubMed).

Walker, Saas, Dietrich: "Role of Fas ligand (CD95L) in immune escape: the tumor cell strikes back." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4521-4, (1997) (PubMed).

Zavazava, Krönke: "Soluble HLA class I molecules induce apoptosis in alloreactive cytotoxic T lymphocytes." in: Nature medicine, Vol. 2, Issue 9, pp. 1005-10, (1996) (PubMed).

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).

Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

Details zu FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: CD178 (FASL Produkte)

Synonyme: ALPS1B antikoerper, APT1LG1 antikoerper, APTL antikoerper, CD178 antikoerper, CD95-L antikoerper, CD95L antikoerper, FASL antikoerper, TNFSF6 antikoerper, fasl antikoerper, Fas-L antikoerper, Faslg antikoerper, Tnfsf6 antikoerper, gld antikoerper, Apt1Lg1 antikoerper, Fasl antikoerper, FASLG antikoerper, zgc:162027 antikoerper, Fas ligand antikoerper, Fas ligand L homeolog antikoerper, Fas ligand (TNF superfamily, member 6) antikoerper, FASLG antikoerper, faslg.L antikoerper, Fasl antikoerper, Faslg antikoerper, faslg antikoerper

Hintergrund: Fas (CD95, APO-1) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or by Fas ligand (FasL, CD178). Fas belongs to the TNF (Tumor Necrosis Factor)/NGF (Nerve Growth Factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. CD178 (FasL), a member of the TNF cytokine family, induces apoptosis by binding to Fas, its cell-surface receptor. FasL may exist as either membrane bound or soluble forms and is expressed by activated T and NK cells. FasL may also be constitutively expressed in some immunologically privileged sites, e.g., eye and testis. Fas and FasL play an important role in the induction of apoptosis, and thus regulate a variety of immunological responses. The NOK-1 antibody clone has been reported to recognize human FasL, recognizing both the membrane bound (FasL) and soluble (sFasL) forms. It is reported that the epitope for NOK-1 has been mapped to the COOH-terminus of FasL, at the region implicated in Fas binding. FasL and sFasL have been reported to migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in the literature. The NOK-1 antibody clone is not recommended for the western blot application.
Synonyms: Fas Ligand, CD95 Ligand

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity