ZAP70 Antikörper (pTyr319) (PerCP-Cy5.5)

Produktnummer ABIN1176924

Produktdetails anti-ZAP70 Antikörper (PerCP-Cy5.5)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Bindungsspezifität: pTyr319

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist konjugiert mit PerCP-Cy5.5

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human phosphorylated ZAP70 Peptide

Klon: 17A-P

Isotyp: IgG1

Anwendungsinformationen

Probenmenge: 5 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

Referenzen für anti-ZAP70 Antikörper (PerCP-Cy5.5) (ABIN1176924)

Di Bartolo, Mège, Germain, Pelosi, Dufour, Michel, Magistrelli, Isacchi, Acuto: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." in: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, (1999) (PubMed).

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, (1994) (PubMed).

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, (1994) (PubMed).

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70 (ZAP70 Produkte)

Synonyme: SRK antikoerper, STCD antikoerper, STD antikoerper, TZK antikoerper, ZAP-70 antikoerper, 70kDa antikoerper, AI327364 antikoerper, Srk antikoerper, mrtle antikoerper, mur antikoerper, zap70 antikoerper, MGC80670 antikoerper, MGC88962 antikoerper, ZAP70 antikoerper, zgc:110383 antikoerper, zeta chain of T-cell receptor associated protein kinase 70 antikoerper, putative tyrosine-protein kinase zap-70 antikoerper, zeta-chain (TCR) associated protein kinase antikoerper, zeta chain of T cell receptor associated protein kinase 70 antikoerper, zeta-chain (TCR) associated protein kinase 70kDa antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa antikoerper, tyrosine-protein kinase ZAP-70 antikoerper, ZAP70 antikoerper, Smp_149470 antikoerper, Zap70 antikoerper, zap70.L antikoerper, zap70 antikoerper, LOC707555 antikoerper, LOC100342021 antikoerper, LOC100390922 antikoerper, LOC100542877 antikoerper

Hintergrund: ZAP70 is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. TCR stimulation leads to autophosphorylation of ZAP70 at Tyr-315 amd Tyr-319, and mutation of the Tyr-319 site dramatically impairs TCR signaling. In addition, TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyr-493 in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. Clone 17A/P-ZAP70 recognizes the phosphorylated form of ZAP70 (Y319). It also cross-reacts with SYK (Y352) due to homology of the phosphorylation site with ZAP70 (Y319). The PE-conjugated format has been evaluated using human and mouse model systems. The unconjugated form of the antibody (Cat. No. 612574) has also been shown to work in western blot analysis on human, mouse, and rat cells.
Synonyms: ZAP-70, SRK, STD, TZK, Zeta-chain associated protein kinase, 70kD

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway