B-Cell Linker Antikörper (N-Term) (Alexa Fluor 647)

Produktnummer ABIN1176912

Produktdetails anti-B-Cell Linker Antikörper (Alexa Fluor 647)

Target: B-Cell Linker (BLNK)

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser B-Cell Linker Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-terminal BLNK Recombinant Protein

Klon: 2B11

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen für anti-B-Cell Linker Antikörper (Alexa Fluor 647) (ABIN1176912)

Taguchi, Kiyokawa, Takenouch, Matsui, Tang, Nakajima, Suzuki, Shiozawa, Saito, Katagiri, Takahashi, Karasuyama, Matsuo, Okita, Fujimoto: "Deficiency of BLNK hampers PLC-gamma2 phosphorylation and Ca2+ influx induced by the pre-B-cell receptor in human pre-B cells." in: Immunology, Vol. 112, Issue 4, pp. 575-82, (2004) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Li, Martin, Oliver, Kearney, Carter: "Antigen receptor proximal signaling in splenic B-2 cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3122-9, (2001) (PubMed).

Minegishi, Rohrer, Coustan-Smith, Lederman, Pappu, Campana, Chan, Conley: "An essential role for BLNK in human B cell development." in: Science (New York, N.Y.), Vol. 286, Issue 5446, pp. 1954-7, (1999) (PubMed).

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: BLNK (BLNK Produkte)

Hintergrund: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The 2B11 monoclonal antibody recognizes BLNK, regardless of phosphorylation status. A fusion protein representing amino acids 4-205 of human BLNK was used as the immunogen. BLNK is expressed as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE that represent alternatively spliced forms of human BLNK.
Synonyms: SLP-65, BASH, BCA

Pathways: BCR Signaling